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Help CenterCapsaicin Elisa Kit for Detecting Oils Item Number：CH97057-2 Brand：Chenhui
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Capsaicin Elisa Test Kit
CH97057-2
Sample Extraction Solution
Weigh 8 g of NaOH, dissolve in 200 mL of deionized water and set aside for later use.
Animal‑ and Vegetable‑Derived Edible Oils (excluding sesame oil)
Weigh 1.0 ± 0.05 g oil sample into a suitable container. Add 1 mL dichloromethane and 2 mL Sample Extraction Solution, then shake for 1 min.
Centrifuge at 4000 rpm for 1 min.
Transfer 1 mL of the upper‑layer liquid, add 0.25 mL Reagent A and 2 mL n‑hexane, then shake for 1 min.
Take 1 mL of upper‑layer n‑hexane, evaporate to dryness, and re‑dissolve with 0.1 mL Sample Diluent.
Dilution factor: 0.4
Sesame Oil
Weigh 0.5 ± 0.05 g oil sample into a suitable container. Add 1 mL dichloromethane and 2 mL Sample Extraction Solution, then shake for 1 min.
Centrifuge at 4000 rpm for 1 min.
Transfer 1 mL of the upper‑layer liquid, add 0.25 mL Reagent A and 2 mL n‑hexane, then shake for 1 min.
Take 1 mL of upper‑layer n‑hexane, evaporate to dryness, and re‑dissolve with 0.1 mL Sample Diluent.
Dilution factor: 1
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