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Help CenterTetrodotoxin(TTX) ELISA Test Kit Brand: Chenhui Model Number:CH98016
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Tetrodotoxin ELISA Kit
CH98016
Tetrodotoxin ELISA Kit provides a competitive enzyme immunoassay for the quantitative analysis of Tetrodotoxin in Pufferfish.Up to 96 tests or assaying about 42 samples in duplicate( assuming 12 wells for standards).
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The unique features of the kit are: 1.Rapid (＜30min), high recovery (≥80%), and cost-effective extraction methods . 2.High sensitivity (1.5ug/kg or ppb) and low detection limit (30 ug/kg orppb) . 3.A quick ELISA assay (less than 1.5 hours regardless of number of samples). 4.High reproducibility. |
Warnings and Precautions |
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1. When compared to quantification by LC/MS or GC/MS, it is not uncommon for immunoassays to report higher analyte concentrations. While LC/MS or GC/MS methods typically measure only a single compound, antibodies used in immunoassays not only recognize the target molecule, but also structurally related molecules, including biologically relevant metabolites. So it is the responsibility of the client to understand the limits of both assays system and to interpret their data accordingly. 2. Any variation in operating procedure, pipetting technique, washing technique, incubating time or temperature etc, can cause variation in result. 3. Do not use the kit beyond the expiration date. 4. Do not mix or substitute reagents with those from other lots or sources. 5. Water used to prepare all ELISA reagents and buffers must be deionized or distilled water. 6. Tetrodotoxin standards contain tetrodotoxin, a neurotoxin, so please be careful in use. Reagent Preparation (1) Extraction-solvent The formulation of Extraction-solvent is as follow: methanol:distilled water:glacial acetic acid=400:599:1; for exemple, methanol 40mL+distilled water 59.9mL +glacial acetic acid 0.1mL (2) 1×sample-diluent Mix 1 volume of 4×sample-diluent with 3 volumes of distilled water.
Pufferfish（muscle, liver, skin） 1. Homogenize a reasonable amount of sample with a suitable mixer. 2. Weigh out 2g of the homogenize sample into a suitable vial and mix thoroughly with 10mL of Extraction-solvent. 3. Keep the vial staying in a 90℃ water bath for 10 minutes and during this procedure, shake the vial for 3 times . 4. Rinse the vial for 1minute with tap water to cool down the sample and then add 1mL of hexane, vortex for 30 seconds; 5. Centrifuge the sample at 4,000×g for 5 minutes, piptte 100μL of the interlayer liquid and add 300μL of 1×sample-diluent 6. Use 50μL of the sample solution per well for the assay. Note: Dilution Factor :20 |
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