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Help CenterTestosterone ELISA Kit Brand：Chenhui ,Model:CH526382
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Testosterone ELISA Kit
CH526382
Testosterone ELISA Kit provides a competitive enzyme immunoassay for the quantitative analysis of Testosterone in Plasma, Serum, milk, fish, shrimp, beef, chicken, pork and urine.Up to 96 tests or assaying about 42 samples in duplicate( assuming 12 wells for standards).
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The unique features of the kit are: 1.Rapid (＜30min), high recovery (≥80%), and cost-effective extraction methods . 2.High sensitivity (0.1 ng/g or ppb) and low detection limit (0.2 ng/g or ppb) . 3.A quick ELISA assay (less than 1.5 hours regardless of number of samples). 4.High reproducibility. |
Warnings and Precautions |
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1. When compared to quantification by LC/MS or GC/MS, it is not uncommon for immunoassays to report higher analyte concentrations. While LC/MS or GC/MS methods typically measure only a single compound, antibodies used in immunoassays not only recognize the target molecule, but also structurally related molecules, including biologically relevant metabolites. So it is the responsibility of the client to understand the limits of both assays system and to interpret their data accordingly. 2. Any variation in operating procedure, pipetting technique, washing technique, incubating time or temperature etc, can cause variation in result. 3. Do not use the kit beyond the expiration date. 4. Do not mix or substitute reagents with those from other lots or sources. 5. Water used to prepare all ELISA reagents and buffers must be deionized or distilled water. |
Meat（Fish,Shrimp,Beef,Chicken,Pork）
1. Homogenize a reasonable amount of sample with a suitable mixer.
2. Weigh out 1g of the homogenize sample into a suitable vial and mix thoroughly with 4mL of Extraction Diluent, vigorously vortex for 2min.
3. Centrifuge it at 4,000 x g for 5 minutes.
4. Use 50μL of the supernatant per well for the assay.
Note: Dilution Factor :4
Milk
1. High-fat milk should be defatted by centrifuging;
2. Pipette 2mL of the milk sample and add 0.5mL of methanol, vortex for 15s
3. Use 50μL per well for the assay.
Note:Dilution Factor:1.25
Urine
1. Centrifuge 1 mL of the urine sample at 4,000 x g for 5 minutes.
2. Pipette 0.5mL of the urine supernatant and mix with 0.4mL of distilled water and 0.1mL of methanol, vortex for 15s;
3. Use 50μL per well for the assay.
Note: Dilution Factor:2
Plasma/Serum
1. Pipette 2mL of the homogenize sample into a suitable vial and mix with 0.5mL of methanol ,vortex for 15s.
2. Use 50μL per well for the assay.
Note: Dilution Factor: 1.25
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