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Help CenterDiazepam (DZP) ELISA Kit Item Number:CH526389 Brand:Chenhui
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Diazepam (DZP) ELISA Kit
CH526389
Principle and Application
This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Diazepam (DZP) in the sample such as Tissue (beef, chicken, pork, etc.), feed. The kit is composed of Microtiter Plate coated with coupled antigens, HRP conjugate, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the DZP in the samples will compete with the coupled antigens to combine with anti-DZP antibodies. After adding HRP conjugate, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with DZP content. Lastly, by comparing the obtained absorbance values with the standard curve, we can calculate the DZP content in the sample.
Storage conditions
l The kit shall be stored at 2-8 ℃. Avoid freezing.
l Shelf life: 12 months. The date of manufacture is presented in the label of the box.
Technique Data
l Kit Sensitivity: 0.3ppb (ng/mL)
l Reactive Mode: 25℃, 30min～30min～15min
l Detection Limits:
Sample |
Detection Limits |
Tissue (beef, chicken, pork, etc.) |
3ppb |
Urine |
3ppb |
Compound feed |
30ppb |
Concentrate/Premix |
60ppb |
l Cross-reaction Rate:
Diazepam…………………………………………………100%
Nitrazepam……………………………………………...＜10%
Oxazepam……………………………………………….＜10%
l Sample Recovery Rate:
Sample |
Recovery Rate |
Tissue |
90±20% |
Urine |
85±20% |
Compound feed |
80±20% |
Concentrate/Premix |
75±20% |
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