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Help CenterCapsaicin Assay ELISA Test Kit Brand: Chenhui Model:CH97057
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Capsaicin Assay ELISA Test Kit Brand: Chenhui Model:CH97057NegotiableMOQ: 1 Box
Capsaicin Assay ELISA Test Kit
CH97057
This product employs competitive enzyme immunoassay technology for the quantitative analysis of the spiciness of peppers and their products. It can accommodate up to 96 tests in total. When conducting duplicate tests, This product supports single-point calibration (i.e., quantitative analysis with one standard), enabling the detection of up to 47 samples. For data analysis with single-point calibration, please contact us to obtain the dedicated Excel data analysis software.
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Product features： l Quickly（＜10min）、High recovery（≥80%）and efficient pre-processing methods l Detection limit: 1 degree of spiciness，Quantitative limit: 2 degrees of spiciness l Fast, detection time 30min l High reproducibility |
Pepper Oil
(1)Weigh 0.5g homogenized sample into a suitable container, add 25mL sample extraction solution, vortex for 2min；
(1) Centrifuge at 4000rpm for 5min；
(2) Take 0.1mL of the supernatant, add 9.9mL deionized water, mix well.
(3) Take 0.1mL of the mixed solution from the previous step, add 0.9mL deionized water, mix well；
(4) Take 0.1mL of the mixed solution from the previous step, add 0.1mL sample diluent, mix well。
Dilution Factor：1
【For samples exceeding the detection limit, an additional step can be added between steps (2) and (3) dilute the liquid obtained in step (2) with methanol to an appropriate multiple before proceeding to the next step; multiply the final result by this multiple】
hotpot condiment
Hot pot base can be heated until melted before weighing; samples with solid particles need homogenization first.
(1) Weigh 3.0g homogenized sample into a suitable container, add 30mL sample extraction solution, vortex for 2min；
(2) Centrifuge at 4000rpm for 5min；
(3) Take 1mL of the supernatant, add 4mL sample extraction solution, mix well；
(4) Take 0.1mL of the mixed liquid, add 9.9mL deionized water, mix well；
(5) Take 0.1mL of the mixed solution from the previous step, add 0.9mL deionized water, mix well；
Take 0.1mL of the mixed solution from the previous step, add 0.1mL sample diluent, mix wel.
Dilution Factor：1
【For samples exceeding the detection limit, an additional step can be added between steps (2) and (3)dilute the liquid obtained in step (2) with methanol to an appropriate multiple before proceeding to the next step; multiply the final result by this multiple】
Dried Pepper
Grind and sieve through a 40-mesh screen.
(1) Weigh 0.5g homogenized sample into a suitable container, add 25mL sample extraction solution, vortex for 2min.
(2) Centrifuge at 4000rpm for 5min
(3) Take 0.1mL of the supernatant, add 9.9mL deionized water, mix well.
(4) Take 0.1mL of the mixed solution from the previous step, add 0.9mL deionized water, mix well.
(5) Take 0.1mL of the mixed solution from the previous step, add 0.1mL sample diluent, mix well.
Dilution Factor：1
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