Diethylstilbestrol (DES) ELISA Kit Brand:Chenhui Item Number:CH526385
Product Spotlights
Diethylstilbestrol (DES) ELISA Kit
CH526385
Principle and Application
This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Diethylstilbestrol (DES) in tissue and other samples. The kit is composed of Microtiter Plate coated with coupled antigens, HRP conjugate, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the DES in the samples will compete with the coupled antigens to combine with anti-DES antibodies. After adding HRP conjugate, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with DES content. Lastly, by comparing the obtained absorbance values with the standard curve, we can calculate the DES content in the sample.
Storage conditions
l The kit shall be stored at 2-8 ℃. Avoid freezing.
l Shelf life: 12 months. The date of manufacture is presented in the label of the box.
Technique Data
l Kit Sensitivity: 0.05ppb (ng/mL)
l Reactive Mode: 25℃, 30min~30min~15min
l Detection Limits:
Sample |
Detection Limits |
Fish, shrimp |
0.2ppb |
Pork, pork liver, chicken, chicken liver |
0.5ppb |
l Cross-reaction Rate:
Diethylstilbestrol …………………………………………100%
Dienestrol………………………………………………...38.5%
Hexestrol…………………………………………………..8.5%
Ethinyl estradiol…………………………………………..
Estriol……………………………………………………..
l Sample Recovery Rate:
Sample |
Recovery Rate |
Tissue |
85±10% |
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