Biological buffer solutions play an important role in biochemical analysis, in vitro diagnostics, and other fields, and CAPSO, as one of the buffer types, is gradually being applied in a wide range of fields. As a zwitterionic buffering agent, CAPSO has a white powder appearance and good solubility in water. Compared with other insoluble buffering agents, CAPSO is more convenient to use. Below, I will introduce it specifically to everyone.
Preparation of CAPSO buffer solution
1. Prepare CAPSO sodium salt powder, distilled water (dH2O), stirring rod, and large beaker in the required proportion for the experiment.
2. Pour CAPSO sodium salt and its corresponding proportion of distilled water into a beaker, and stir with a stirring rod until the CAPSO sodium salt powder is dissolved.
3. Next, it is necessary to adjust the pH value to the desired level, and then pour distilled water (dH2O) to the required volume.
4. By filtering, disinfecting, and sterilizing, CAPSO finished solution can be obtained.
Application of CAPSO buffer solution
1. It is often used in enzyme catalyzed reactions and the preparation of some detection reagents, or in immunoblotting and precipitation experiments.
2. CAPSO can be used as a buffer to prevent significant changes in pH, maintain ion strength within a reasonable range, and prevent protein from binding with other ions to form insoluble products.
3. It can provide a good storage condition for various experimental solutions, which is conducive to stable and effective storage, and avoids adverse reactions.
4. It can be used in medical diagnostic kits and has significant clinical significance for the occurrence and treatment of diseases such as liver disease and myocarditis.
Precautions for using CAPSO buffer solution
1. Due to the fact that the CAPSO produced is in powder form, it is important to avoid moisture during use. It should be stored in a sealed container at room temperature and immediately sealed after use. If it is a pre prepared solution, it needs to be stored at a low temperature of 4 degrees Celsius.
2. In order to achieve better buffering effect of CAPSO, it is recommended to use it immediately to avoid long-term storage, which may cause deterioration and affect the experimental detection process.
3. During the experiment, it is necessary to take protective measures, wear protective gear, and avoid direct contact with the skin. If accidentally touching the skin, rinse it off with running water in a timely manner.
Desheng is a direct supplier of biological buffer solutions, specializing in research, development, production, and sales. Not only does it ensure purity, but it also has control over the process and raw materials. Even if there are problems, it can provide solutions in a timely manner and offer customers one-stop services. If you have any relevant intentions, please feel free to call us for more information!
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