Preparation and Use of New Trinder's Reagent DAOS 83777-30-4 Solution

The new Trinder's reagent DAOS (3,5-Diacetyl-1,4-dihydrolutidine), CAS number 83777-30-4, has the advantages of high water solubility and sensitive reaction, so it is commonly used in clinical testing kits, such as uric acid and renal function tests. As the finished product produced is a powder, it needs to be prepared into a solution when used, and many people are not clear about the specific operation and some precautions. Therefore, the following will introduce it to everyone in detail.

Method for preparing DAOS solution

1. Dissolve DAOS powder in the calculated PBS buffer, and adjust the specific solubility as needed.

2. 
   

2. Dissolve 4-aminoantipyrine (4-AA) in PBS buffer to prepare a 4-AA solution.

3. Prepare a 2 U/ml horseradish peroxidase solution using PBS.

4. Then mix the solutions of equal capacity together to prepare the detection solution.

Precautions for using DAOS reagents

1. Safe operation: When preparing and using DAOS reagents, it is necessary to strictly follow the formal experimental procedures, wear personal protective equipment such as gloves, goggles, and professional jackets, and avoid direct contact with skin, eyes, and clothing to avoid irritation or injury.

2. 
   

2. Storage conditions: Store DAOS reagents in dry, dark, and sealed containers to prevent moisture and light exposure. It is recommended to store it in a low-temperature environment, such as refrigeration or freezing, to extend its stability and service life.

3. Reagent stability: DAOS reagents can maintain stability for a long time under appropriate storage conditions, but the following points still need to be noted:

(1) Avoid exposure to high temperature environments: High temperatures may cause the decomposition or degradation of reagents, so prolonged exposure to high temperatures should be avoided as much as possible.

(2) Preventing moisture intrusion: Moisture may cause degradation and agglomeration of reagent powder, so it is important to ensure a sealed container before and after use and avoid environments with high humidity as much as possible.

(3) Pay attention to lighting conditions: DAOS reagents are sensitive to light, so they should be stored in a dark place and avoid prolonged exposure to strong light or ultraviolet rays as much as possible.

4. Optimization of experimental conditions: When using DAOS reagents for analysis, it is necessary to optimize the conditions according to specific experimental requirements, including pH value, temperature, reaction time, and concentration of DAOS reagents. The optimization of these conditions can improve the detection sensitivity and accuracy of the reagents.

5. Operation skills: When using DAOS reagents, it is necessary to avoid contact with other chemicals to prevent possible interference reactions. At the same time, ensuring the correct order of reagent addition and uniform mixing is crucial for accurate results.