The Buffering Range of GOOD'S Buffer EPPS 16052-06-5

In the fields of biochemistry and molecular biology, the use of appropriate buffering agents is of great significance for maintaining the stability of reaction systems. Among them, EPPS is a commonly used GOOD'S buffer, which is a buffer containing a piperazine ring structure that endows EPPS with superior buffering performance. Its molecular formula is C9H20N2O4S, with a relative molecular weight of 252.33 g/mol. It is soluble in water, making it suitable for buffering requirements under many experimental conditions. What is its buffering range and what is its function? Let's take a look below.

1、 EPPS buffer range

The pH range of buffering agents is usually defined as the range within which they can stably maintain the pH of the solution and prevent drastic changes in pH. As a buffer, EPPS has good buffer performance in a specific pH range of 7.3-8.7, and Pka value is 8.0 at 25 ℃. This range is suitable for many biochemical and molecular biological experiments, such as ultra-thin isofocal gel as a separator and as a buffer component of RNA samples.

2、 EPPS buffer range performance advantage

1. Stability: EPPS exhibits excellent stability within its buffering range, and during the experimental process, it can effectively maintain the required acid-base balance when the pH value of the solution needs to be kept constant.

2. Electrophoresis application: EPPS buffer is also commonly used in DNA/RNA electrophoresis experiments, especially in the process of preparing nucleic acid samples. It can provide stable electrophoresis conditions to ensure that nucleic acids move at an appropriate rate in gel.

3. Protein stability: Within the pH range of 7.3-8.7, EPPS buffer can provide an appropriate environment, such as protecting oxygenated hemoglobin from oxidation to methemoglobin during freeze-drying.

The buffering range of EPPS buffer covers many acid-base conditions required for experiments, ensuring the accuracy of experimental results. Regardless of the field, it exhibits excellent performance advantages and provides effective experimental conditions for experimenters. As an experienced manufacturer of buffering agents, Desheng can provide raw material powders with a purity of up to 99%, good water solubility, and a complete range of product types, with personalized customized services according to customer requirements. For ton level purchasing customers, price adjustments are flexible. If you have any relevant intentions, please feel free to contact us for purchase at any time!