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Brand:MOTOROLA
Type:MVME2434
Origin:the United States
Warranty: 365 days
Colour: new/used
Shipping method: Courier delivery
Products are widely used in metallurgy, petroleum, glass, aluminum manufacturing, petrochemical industry, coal mine, papermaking, printing, textile printing and dyeing, mechanical, electronic manufacturing, automobile manufacturing, plastic machinery, electric power, water conservancy, water treatment/environmental protection, boiler heating, energy, power transmission and distribution and so on.
Spare parts spare parts, the main DCS control system of PLC system and the robot system spare parts,
Brand advantage: Allen Bradley, BentlyNevada, ABB, Emerson Ovation, Honeywell DCS, Rockwell ICS Triplex, FOXBORO, Schneider PLC, GE Fanuc, Motorola, HIMA, TRICONEX, Prosoft etc. Various kinds of imported industrial parts
Buffer Preparation P Before starting: Add 24 ml 100% ethanol (26 ml 95% ethanol) to the 6 ml DNA Wash Buffer concentrate. Add 96 ml 100% ethanol (104 ml 95% ethanol) to the 24 ml DNA Wash Buffer concentrate. P DNA Wash Buffer included with D4001S and D4001T is supplied ready-to-use and does not require the addition of ethanol prior to use. Sample Processing All centrifugation steps should be performed between 10,000 - 16,000 x g. 1. In a 1.5 ml microcentrifuge tube, add 2-7 volumes of DNA Binding Buffer to each volume of DNA sample (see table below). Mix briefly by vortexing. Application DNA Binding Buffer : Sample Example Plasmid, genomic DNA (>2 kb) 2 : 1 200 µl : 100 µl PCR product, DNA fragment 5 : 1 500 µl : 100 µl ssDNA1 (e.g. cDNA, M13 phage) 7 : 1 700 µl : 100 µl For efficient recovery of genomic or large DNA (> 20 kb to > 200 kb), use the Genomic DNA Clean & Concentrator® (Cat. Nos. D4010, D4011). 2. Transfer mixture to a provided Zymo-Spin™ Column2 in a Collection Tube. 3. Centrifuge for 30 seconds. Discard the flow-through. 4. Add 200 µl DNA Wash Buffer to the column. Centrifuge for 30 seconds. Repeat the wash step.
1 For ssDNA purification, see Appendix A on page 10. 2 The sample capacity of the column is 800 µl. Therefore, it may be necessary to load and spin a column multiple times if a sample has a volume larger than 800 µl. 7 5. Add ≥ 6 µl DNA Elution Buffer3 or water4 directly to the column matrix and incubate at room temperature for one minute. Transfer the column to a 1.5 ml microcentrifuge tube and centrifuge for 30 seconds to elute the DNA. Ultra-pure DNA is now ready for use.3 DNA Elution Buffer: 10 mM Tris-HCl, pH 8.5, 0.1 mM EDTA 4 Elution of DNA from the column is dependent on pH and temperature. If water is used, make sure the pH is >6.0. Waiting 1 minute prior to elution may improve the yield of larger (> 6 kb) DNA. For even larger DNA (> 10 kb), the total yield may be improved by eluting the DNA with 60-70o C DNA Elution Buffer.
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