DW-K5500 micro-spectrophotometer
DW-K5500 is a new type full - spectrum ( 200~ 850nm) spectrophotometer, can measure nucleic acids, proteins, cell cultures, microarray samples, and UV-VIS full spectrum absorbance scan with high accuracy and reproducibility.
Features
0.3~2µL sample size!
No cells, no cuvettes!
No warm-up!Rapid measure speed!
Display the concentration directly!
UV-VIS full spectrum absorbance analysis (200-850nm)!
No dilutions for samples, can measure samples with high concentration!Easy to use!
Performance index
Path length: 1mm, 0.2mm, (0.1mm, 0.05mm optional) Sample size: 0.3~2µL Light source: Xenon flash lamp Detector: 2048-element linear silicon CCD Wavelength range: 200~850nm Wavelength accuracy: ±1nm Wavelength resolution: 2nm (FWHM at Hg 546nm) Absorbance precision: 0.002 Abs Absorbance accuracy: 1% (0.76 absorbance at 350nm) Absorbance range: 0.02~75(150, 300 optional, 10mm equivalent absorbance) Detects Nucleic acid up to: 2~3750ng/μL(7500,15000 optional, dsDNA) Detects Protein up to: 0.1mg/ml~100mg/ml(200,400 optional, BSA) Measurement time: <5s Outline size: 21cm×17cm×11cm Net weight: 1.35kg
Application
There are 5 measurement modules provided by K5500 software: Nucleic acid, Protein, Cell culture, Microarray and UV-VIS.
Nucleic Acid: Concentration and purity of DS-DNA, SS-DNA and RNA
Protein: Concentration of the protein A280(1Abs = 1mg/mL), BSA, IgG and lysozyme Concentration of protein with protein assay kit (Lowry, BCA and Bradford)
Cell Culture:Concentration of suspended cell cultures
UV-VIS: UV/VIS full-spectrum (200~850nm) absorbance scan
Microarray:Concentration of fluorescent dye labeled nucleic acid (microarray), for it can measure the concentration of the nucleic acid and the dye at the same time
General operation
1 Drop directly 0.3~2µL sample onto the test platform with a pipette.
2 Close the sampling arm.
3 Click the “Measure” button, and the result(values and graph) of the sample will appear in seconds.
4 Wipe the sample from the test platform with clear absorbent paper.
5 Click the “Save Graphic” button after measured a sample if you want to save the graph.
6 Click the “Show Report” button after the samples measured to output the result data of the samples by excel.
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