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Introduction
The high-throughput strain screening and optimization system is a multi-channel miniature bioreactor with pH detection, control, and feeding functions based on a shaker. This reactor is an essential special equipment in the bioreaction process. It is used for microbial, animal, and plant cell strain screening, physiological characteristic research, medium optimization, seed expansion culture, etc., especially in early large-scale research work of various microorganisms and genetically engineered bacteria, its effect is significantly better than conventional shakers. It is widely used in biochemistry, medicine, food, light industry, environmental protection, pesticide and other fields.
Main Advantages
l Real-time pH changes can cause changes in the physiological characteristics of strains, and the pH detection function provides strong evidence for metabolic flux analysis.
l The carefully designed micro-feeding technology combined with real-time pH detection and fed-batch technology is of great significance for achieving dynamic research with metabolic flux as the core.
l The comparative operation of multi-channel shaking flasks and various combinations of processes can greatly shorten the progress of process research.
l Simultaneous aeration of CO, O2, and N, and multiple culture modes are possible.
Main Applications
1. Research on the pH change law of bacterial growth process, inoculation amount, and inoculation timing
2. Optimization of basic mediumResearch on the optimal pH for bacterial growth stage and product formation stage
3. Research on fed-batch feeding, including feeding type, rate, and kinetic research
4. Realization of dynamic research with metabolic flow as the core
Scalable Configuration
LED lighting system (algae/plant cell culture) |
Exhaust gas O/CO online detection |
Humidity detection |
Alcohol content online detection |
Feeding weighing |
Cell concentration online detection |
ORP/OD detection |
Viscous tray can work without flask clamp |
Technical Indexes
Name Technical Indexes Configuration and Description |
||
Cabinet Size |
850×620×1355(mm) |
Professional design |
Rocking plate size |
760×460(mm) |
Stainless steel 316L |
Eccentric distance |
20(Single side) |
|
Special Shaking Flask |
250mL/500mL/1000mL |
Specially made with feeding port, aeration interface, ventilation port, sensor socket, baffle |
Temperature System |
Control Range：5-50℃ Control Accuracy：±0.2℃ |
PT100 probe, (with refrigeration system) |
Speed System |
Speed：0-300rpm Accuracy：±2% |
DC speed regulating motor, stepless speed regulation, photoelectric speed measurement, low noise, low vibration |
Feeding System |
Range: 0.2ml~200ml/min Accuracy：±0.02ml |
The micro-peristaltic pump allows for exponential and acceleration feeding modes. It also supports single-point calibration by replacing silicone tubing of different diameters. It also features metering, accumulation, and data clearing. |
Aeration System |
Range：1-20L/min Accuracy：±5% |
Dedicated ventilation valve |
|
pH control system |
Range：2-12 Accuracy：±0.01 |
Customizable control strategies can be developed, such as through equations, exponential models, and other control modes. Supports single-point calibration after sterilization. pH electrode life display. Single-point calibration is available after replacing silicone tubing of different diameters. Features include metering, accumulation, and data clearing. |
Channel |
6、8、12、16、18 |
The software uses exhaust CO₂/O₂ concentration data to calculate OUR (oxygen uptake rate)/CER (CO₂ release rate) and RQ (respiratory quotient) online
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