Deoxynivalenol (DON) ELISA Test Kit
Deoxynivalenol (DON) ELISA Test Kit

l The kit shall be stored at [2-8 ℃]. Expiry date: 12 months
l The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
IntroductionThe test kit is detecting for the quantitative analysis of Deoxynivalenol in cereals (rice, millet, noodle and etc), feed and other sample.
Principle of testThe Deoxynivalenol (DON) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The DON antigen has been coated in the plate wells. During the analysis, sample is added along with the DON-horseradish peroxidase (DON-HRP) conjugate. If the DON residue is present in the sample, it will compete for the DON antibody, thereby preventing the DON-HRP from binding to the antibody attached to the well, last, take coloration with TMB substrate. The samples absorbance value is an inverse relationship with DON content. Lastly, compared with the standard curve can be concluded the DON residues in sample.
Technique Datal Kit sensitivity: 3ppb (ng/ml)
l reactive mode: 37℃, 30min~30min~15min
l Detection Limits:
Sample |
Detection Limits |
Cereals |
150 ppb |
Feed. |
150 ppb |
l Cross-reaction rate:
Deoxynivalenol ………………………….100%
3- acetyl-DON …………………………..
l Sample Recovery rate:
Cereal …………………………………..……. 85±15%
Compound feed …………………………………….. 85±15%
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