Chloramphenicol (CAP) ELISA Test Kit
Chloramphenicol (CAP) ELISA Test Kit
Storage conditionsl The kit shall be stored at [2-8 ℃]. Expiry date: 12 months
l The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
IntroductionThe test kit is detecting for the quantitative analysis of Chloramphenicol in honey, aquatic product, tissue, eggs, liver, milk, feed and etc.
Principle of testThe Chloramphenicol (CAP) ELISA Test Kit is using indirect competitive enzyme immunoassay method.
The CAP coupling antigen has been coated in the plate wells. During the detection, add Standards or samples to the well, CAP of samples will compete with coated CAP coupling antigen to combine with antibody, after adding antibody working solution, Horseradish Peroxidase (HRP), take coloration with TMB substrate. The samples A value is an inverse relationship with CAP residue content. Lastly, compared with the standard curve can be concluded the CAP residues in sample.
Technique Datal Kit sensitivity: 0.05 ppb(ng/ml)
l reactive mode: 25℃, 30min~30min~15min
l Detection Limits:
Sample |
Detection Limits |
Tissue, Liver, Honey, Milk |
0.025 ppb |
Eggs |
0.1 ppb |
Urine, Serum, Casing, Feed, Milk powder |
0.05 ppb |
l Cross-reaction rate:
Name |
Cross-reaction rate |
Chloramphenicol |
100% |
Thiamphenicol |
<0.1% |
Florfenicol |
<0.1% |
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