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Aflatoxin M1 (AFM1) ELISA Test Kit
Storage conditionsl The kit shall be stored at [2-8 ℃]. Expiry date: 12 months
l The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
IntroductionThe test kit is detecting for the quantitative analysis of Aflatoxin M1 in milk, milk powder.
Principle of TestThe Aflatoxin M1 (AFM1) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the toxin coated on the plate wells, last, take coloration with TMB substrate. The samples absorbance value is an inverse relationship with AFM1 content. Lastly, compared with the standard curve can be concluded the AFM1 residues in sample.
Technique Datal Kit sensitivity: 0.02ppb (ng/ml)
l reactive mode: 25℃, 30min～15min
l Detection Limits:
Sample |
Detection Limits |
Milk |
0.04 ppb |
Milk powder |
0.06 ppb |
l Cross-reaction rate:
Aflatoxin M1 ………………………….100%
l Sample Recovery rate:
Milk …………………………………..……. 85±15%
Milk powder …………………………………….. 80±15%
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