Salbutamol ELISA Test Kit

Salbutamol ELISA Test Kit

Catalog No. LSY-10014

1. Principle

The test kit is based on the competitive enzyme immunoassay for the detection of Salbutamol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Salbutamol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Salbutamol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Salbutamol in it. This value is compared to the standard curve and the Salbutamol residues is subsequently obtained.

2. Technical specifications

Sensitivity:  0.1 ppb

Incubator temperature:  25℃

Incubator time:  30min~15min

Detection limit

Urine……………………………………………………………..…..…..….0.1 ppb

Tissue (method one)..................................................................... 0.4 ppb

Tissue (method two)...................................................................... 0.1 ppb

Feed............................................................................................... 1 ppb

Recovery rate

    Urine ………………………………………………………………………95%±17%

Tissue.......................................................................................... 75±22%

Feed............................................................................................ 80±18%

Cross-reaction rate

Salbutamol...................................................................................... 100%

Clenbuterol........................................................................................ <1%

Terbutalin.......................................................................................... <1%

Ractopamine................................................................................. < 0.1%

3. Components

1)   Micro-well strips: 12 strips with 8 removable wells each

2)   6× standard solution (1 mL each): 0 ppb, 0.1ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb,8.1 ppb

3)   Enzyme conjugate (7 mL)............................................................... red cap

4)   Antibody working solution (10 mL)................................................ blue cap

5)   Substrate A solution (7 mL)......................................................... white cap

6)   Substrate B solution (7 mL)......................................................... black cap

7)   Stop solution (7 mL).................................................................. yellow cap

8)   2× concentrated redissolving solution (50 mL) ……………………..white cap

9)   20x concentrated washing buffer (40 mL) .......................... transparent cap

10)20x Sample extraction solution (50 mL)  ……………….………..yellow cap