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Nitrofuran (AHD) ELISA Test Kit
Catalog No. LSY-10003
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Aminohydantion (AHD) in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Aminohydantion (AHD) in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AHD antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AHD in it. This value is compared to the standard curve and the AHD concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05 ppb
Incubation Temperature: 37℃
Incubation Time: 30min~30min~15min
Detection limit
Tissue, Liver················································································· 0.1 ppb
Milk, intestine, honey····································································· 0.1 ppb
Fish, shrimp (some interference) ·················································· 0.15ppb
Cross-reaction rate
AHD································································································ 100%
AMOZ··························································································· ﹤0.1%
AOZ······························································································ ﹤0.1%
SEM····························································································· ﹤0.1%
Recovery rate
Tissue, liver·················································································· 85±25%
Honey, milk, intestine···································································· 80±18%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb and 4.05ppb
3) Enzyme conjugate (12 mL)····························································· red cap
4) Antibody working solution (7 mL)·················································· blue cap
5) Substrate A solution (7 mL)························································· white cap
6) Substrate B solution (7 mL)························································ black cap
7) Stop solution (7 mL)································································· yellow cap
8) 20× concentrated washing buffer (40 mL)···································· white cap
9) 2× concentrated redissolving solution (50 mL)···················· transparent cap
10) 2-Nitrobenzaldehyde (10 mL)······················································· white cap
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