Nitrofuran (AHD) ELISA Test Kit

Nitrofuran (AHD) ELISA Test Kit

Catalog No. LSY-10003

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Aminohydantion (AHD) in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Aminohydantion (AHD) in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AHD antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AHD in it. This value is compared to the standard curve and the AHD concentration is subsequently obtained.

2. Technical specifications

Sensitivity:  0.05 ppb

Incubation Temperature:  37℃

Incubation Time:  30min~30min~15min

Detection limit

Tissue, Liver················································································· 0.1 ppb

Milk, intestine, honey····································································· 0.1 ppb

Fish, shrimp (some interference) ·················································· 0.15ppb

Cross-reaction rate

AHD································································································ 100%

AMOZ··························································································· ﹤0.1%

AOZ······························································································ ﹤0.1%

SEM····························································································· ﹤0.1%

Recovery rate

Tissue, liver·················································································· 85±25%

Honey, milk, intestine···································································· 80±18%

3. Components

1)    Micro-well strips: 12 strips with 8 removable wells each

2)    6× standard solution (1 mL each): 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb and 4.05ppb

3)    Enzyme conjugate (12 mL)····························································· red cap

4)    Antibody working solution (7 mL)·················································· blue cap

5)    Substrate A solution (7 mL)························································· white cap

6)    Substrate B solution (7 mL)························································ black cap

7)    Stop solution (7 mL)································································· yellow cap

8)    20× concentrated washing buffer (40 mL)···································· white cap

9)    2× concentrated redissolving solution (50 mL)···················· transparent cap

10)   2-Nitrobenzaldehyde (10 mL)······················································· white cap