Tylosin ELISA Test Kit

Tylosin ELISA Test Kit

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Tylosin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tylosin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tylosin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tylosin in it. This value is compared to the standard curve and the Tylosin concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 1.5 ppb

Detection limit

Meat, liver........................................................................................ 3 ppb

Honey........................................................................................... 1.5 ppb

Recovery rate

Meat............................................................................................. 90±15%

Liver............................................................................................. 80±10%

Honey.......................................................................................... 85±15%

3. Components

1)   Micro-well strips: 12 strips with 8 removable wells each

2)   6× standard solution (1 mL each): 0 ppb, 1.5 ppb, 4.5 ppb, 13.5p pb ,40.5 ppb,121.5 ppb

3)   Enzyme conjugate (7 mL)............................................................... red cap

4)   Antibody working solution (7 mL).................................................. blue cap

5)   Substrate A solution (7 mL).......................................................... white cap

6)   Substrate B solution (7 mL)......................................................... black cap

7)   Stop solution (7 mL).................................................................. yellow cap

8)   20× concentrated washing buffer (40 mL)..................................... white cap

9)   2× concentrated redissolving solution (50 mL)..................... transparent cap