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Tylosin ELISA Test Kit
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Tylosin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tylosin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tylosin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tylosin in it. This value is compared to the standard curve and the Tylosin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 1.5 ppb
Detection limit
Meat, liver........................................................................................ 3 ppb
Honey........................................................................................... 1.5 ppb
Recovery rate
Meat............................................................................................. 90±15%
Liver............................................................................................. 80±10%
Honey.......................................................................................... 85±15%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 1.5 ppb, 4.5 ppb, 13.5p pb ,40.5 ppb,121.5 ppb
3) Enzyme conjugate (7 mL)............................................................... red cap
4) Antibody working solution (7 mL).................................................. blue cap
5) Substrate A solution (7 mL).......................................................... white cap
6) Substrate B solution (7 mL)......................................................... black cap
7) Stop solution (7 mL).................................................................. yellow cap
8) 20× concentrated washing buffer (40 mL)..................................... white cap
9) 2× concentrated redissolving solution (50 mL)..................... transparent cap
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