1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of semicarbazide
(SEM) in chicken, fish, shrimp, milk, honey, whole egg. The coupling antigens are pre-coated on
the micro-well stripes. The SEM in the sample and the coupling antigens pre-coated on the microwell
stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the
TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative
correlation with the SEM in it. This value is compared to the standard curve and the SEM
concentration is subsequently obtained.
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