ELISA
Test
Kit
1.
Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Tetracyclines in
the sample. The coupling antigen s are pre-coated on the micro-well stripes. The Tetracyclines in
the sample and the coupling antigen s pre-coated on the micro-well stripes compete for the anti-
Tetracyclines antibody. After the addition of the enzyme conjugate, the TMB substrate is added for
coloration. The optical density (OD) value of the sample has a negative correlation with the
Tetracyclines in it. This value is compared to the standard curve and the Tetracyclines
concentration is subsequently obtained.
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