ELISA
test
Kit
1.
Principle
This test kit is based on the competitive enzyme immunoassay for the detection of
Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The
Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes
compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the
TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative
correlation with the Chloramphenicol in it. This value is compared to the standard curve and the
Chloramphenicol concentration is subsequently obtained.
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