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Help CenterPerformance Characteristics and Usage Instructions of Color Developing Substrate DA67 115871-18-6
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As a water-soluble chromogenic reagent, DA67 not only has improved water solubility and sensitivity compared to traditional reagents, but also is simple and fast. Results can be obtained directly using a colorimeter, making it very suitable for the detection of biological component samples. Insiders have a clear understanding of it, but outsiders may not know much about it. Therefore, below is a general introduction to its characteristics and usage.
CHEMICAL
The chromogenic substrate DA67, with a CAS number of 115871-18-6 and a molecular weight of 408.45, appears as a white crystalline powder that is soluble in water. Its chromogenic reaction is usually produced by coordination with peroxidase. For the first time, DA67 is compatible with water solubility and high sensitivity. Previously, it was necessary to use luminescent or fluorescent reagents to achieve the goal, but now it can be done using DA67 without the need for other luminescent agents.
application area
Because it can directly obtain results using a colorimeter, it is suitable for detecting biological component samples, such as protein electrophoresis, nucleic acid electrophoresis, and other experiments as a substrate. By measuring the time of substrate consumption and changes in absorbance, information such as enzyme activity and substrate concentration can be reflected.
Performance characteristics
1. High sensitivity: DA67 has high sensitivity and can detect small changes in substrate consumption, which makes it widely used in biochemical experiments and molecular biology.
2. Good repeatability: The color reaction of DA67 has good repeatability and can be quantitatively analyzed through standardized methods, which makes the experimental results more accurate and reliable.
3. High water solubility: Traditional water-soluble color reagents, due to the low molecular absorption coefficient of color pigments, are prone to micro solubility that is difficult to handle. However, DA67 has high water solubility and can be directly dissolved in water at concentrations of 1mmol/L.
precautions
1. DA67 is easily affected by light and heat, so it is necessary to avoid prolonged exposure to air or high temperatures during storage and use. It is recommended to store it in a cool and dry place and use it as soon as possible.
2. The color reaction of DA67 is influenced by various factors, including substrate concentration, enzyme activity, reaction temperature, etc. Therefore, reasonable adjustments and controls need to be made according to experimental requirements during use.
3. When measuring the absorbance of DA67, it is necessary to calculate and select the appropriate detection wavelength to ensure the accuracy and reliability of the results, while also paying attention to calibrating the instrument and eliminating external interference.
As an advantageous supplier of chromogenic substrates, Desheng not only has DA67, but also products such as TOOS, TOPS, ADOS, ADPS, etc. Its sensitivity and reagent stability have been highly praised by customers. We are currently selling at a discounted price. If you are interested, please click on the website to inquire and purchase!
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