Real Time RT-PCR Machine
| Real-Time Quantitative PCR | ||||
| Gentier 96E | Gentier 96S | Gentier 96R | Gentier 96C | |
| Thermal Block | ||||
| Sample capacity(wells) | 96 | |||
| Reaction Volume | 0-100 μL | |||
| Consumables | 0.2ml 96-well Plates(unskirt); 0.2ml 8-strip tubes ,0.2ml PCR single tube(Optical flat cap,clear,frosted,white tube) | |||
| Temperature range | 0°C-100°C | |||
| Heating/cooling method | Peltier | |||
| Max heating rate | 6.1°C/sec | |||
| Average heating rate | 4.5°C/sec | |||
| Max cooling rate | 5.0°C/sec | |||
| Average cooling rate | 2.8°C/sec | |||
| Temperature accuracy | ±0.1℃ | |||
| Temperature uniformity | ±0.1℃ | |||
| Gradient Range | 1°C-40°C | N/A | 1°C-40°C | N/A |
| Gradient block | 12 row | 12 row | ||
| Special temperature portocol | Gradient PCR,Long PCR,Touch Down PCR | Long PCR,Touch Down PCR | Gradient PCR,Long PCR,Touch Down PCR | Long PCR,Touch Down PCR |
| Heat Lid | ||||
| Temperature range | Room Temperature–110°C | |||
| Optical System | ||||
| Excitation Source | 6 LEDs | 4LEDs | ||
| Detector | Photodiode | |||
| Detection Position | Excitation and scan at top | |||
| Detection method | 6 channels scanning at the same time, no edge effect. | |||
| Detection time | 7 seconds for 96 wells for all channels | |||
|
Range of excitation/ emission wavelengths (nm) |
1 465 /510 (FAM,SYBR Green I,SYTO9,EvaGreen,LC Green) 2 527/ 563 (HEX,VIC,TET,JOE) 3 580 /616 (ROX,Texas Red) 4 632/ 664( Cy5) 5 680 /730 (Alexa Fluor680) 6 465 /616 (FRET) |
1 465 /510 (FAM,SYBR Green I,SYTO9,EvaGreen,LC Green) 2 527/ 563 (HEX,VIC,TET,JOE) 3 580 /616 (ROX,Texas Red) 4 632/ 664( Cy5) |
||
| Probe | Taqman Probe,Molecular beacons probe,Scorpion probe ,FRET | Taqman Probe,Molecular beacons probe,Scorpion probe | ||
| Multiplexing | Up to 6 targets | Up to 4 targets | ||
| Fluorescence Linearity | r≥0.990 | |||
| Fluorescence Dynamic Range | Adjustable | |||
| Performance | ||||
| Sample Linearity | /r/≥0.999 | |||
| Sample repeatability | Ct value CV≤0.5% | |||
| Sample Dynamic Range | 1-1010 copies | |||
| Software Funtions | ||||
| Data Analysis modes |
Qualitative analysis,Absolute quantification, Relative quantification,Genotyping analysis,Endpoint analysis, Melt curve analysis, High Resolution Melting |
|||
| Control modes |
1.10.4' touch screen 2. PC direct control 3. WLAN control(One PC can control max 10 units, and device can be controled by any PC in the WLAN) |
|||
| Sample Drawer | Touch sreen commend | |||
| Data Stroage | Upload and download through USB disk,1000 results can be stored in machine | |||
| Power failure protection | Automaticly start running experiments after power supply, no need wait PC software | |||
| Customize Report | Templetes reserved ,report can customized | |||
| Administration Management | Administrator can set funtions limts for users | |||
| Transport Locker | Automaticly detect transport locker | |||
| Fault Management | Fault report and analysis, solution instruction | |||
| LIS connection | CSV,Excel,TXT format data output,open port for LIS connection | |||
| Others | ||||
| PC operating system | Win 7, Win 10 | |||
| Communication Port | 1 ethernet and 3 USB | |||
| Footprint(WxDxH) | 355mm X 480mm X 485mm | |||
| Weight | 30kg | |||
| Power usage | AC 100 to 125 V / 200 to 240 V (50/60 Hz). | |||
| power consumption | 900VA | |||
| Work environment | Tempereture:10°C~30°C Humidity:20%~85% | |||
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