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Real-time Quantitative PCR Detection System kit
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Product Details
| Brand Name | Drawell | Place of Origin | Shanghai, China | |
| Model Number | DW-TL988-IV |
Product Description
Real-time Quantitative PCR Detection System DW-TL988-IV
Design for flexible use
uides you with ease through plate setup.
The DW-TL-988 system adapts to almost any workflow requirement with flexible instrument control and data management.
>> Standard with 7’’ LCD touch screen, functions quickly accessible through simple, one-touch commands
>> High-throughput up to 96 samples per run
>> Push-pull, electrical controlled elevating platform
>> Concealed lid with constant temperature and isopiestic pressure bring high accurate temperature control
>> Quick-start set-up so you can begin a run immediately
>> Unique power-off protection function can save all the setted configurations for sudden power outages, and allow the experiment continues when power supply is restored.
>> System can be installed in three distinct configurations, providing unmatched flexibility and convenience.
1. Co-located configuration (PC control)
2. Standalone (PC-free) configuration (Data can be export by USB and analysis on PC)
3. Networked configuration (PC software automatically detect all systems in LAN and allow to remote monitoring one of experimental progress and downloading of the completed run file to the PC at your desk)
Fast and uniform heating and heat sinking block
>> Concealed lid with constant temperature and isopiestic pressure
>> Electrical controlled elevating & automatic locked platform
>>Thermo block is based on improved heat pipe technology, leading to extraordinary Iintra-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility.
>> Highly efficient PID&DSP control algorithm ensures faster respond and stronger interference resistance, fast and uniform heating and heat sinking
High sensitive and precise optical system
>> High-sensitivity and wide dynamic range of fluorescence detection system, sample dynamic range could be from 101 to 1010 copies
>> Fast and precise mechanical scanning structure ensures the illumination uniformity of 96 wells, Eliminate edge effects, no need for ROX calibration
>> Fluorescence excitation by a high-intensity and long-life LED, Bulb Luminance will not attenuation over time, free maintenance optical system through the entire life.
>> Professional designed fluorescence filters realize the highly fluorescence transmittance and meanwhile minimize the fluorescence channel crosstalk.
Easy-to-use Software
The TL-988 Software offers an intuitive experience for protocols programming, plate layouts setting and experimental results analysising. The Software includes an unlimited license for multiple users. The available software modules include Absolute quantification, Melting curve analysis, Relative quantification and High Resolution Melting (HRM). All the excellent features make the instrument to your ideal choice for qPCR.
>> Friendly interface and clearly functional modules design, first-time users can easily start a new experiment.
>> All experiment data and setting options are shown conveniently on a single interface. User modifications to analysis parameters can be seen instantly
>> Powerful analytical capabilities, optional automatic and manual threshold setting bring reliable date you can work on.
>> Experimental date can be export in .csv, .excel, .txt format, with this capability, the TL-988 can interface with your laboratory information system directly.
>> Unique designed universal report can be customized according to the requirements, report review and print function help clinical documentation as needed.
Generate the quality data that you can depend on
Excellent amplification and homogeneity of reaction products
This real-time PCR run was done using 5 ng of hepatitis B virus DNA in each of the 96 block positions. (A) A 135 bp amplicon of the hepatitis B virus gene was amplified using SYBR Green I detection. (B) The reaction product was also subjected to melting curve analysis. In summary, low variation Cq values (Cq range = 0.15and SD = 0.040) and overlapping melt curves of the amplicon (Tm range of 0.5°C and SD = 0.065) in each of the 96 positions demonstrated temperature homogeneity and equal treatment of all samples - independent of block position.
A hepatitis B virus gene fragment was amplified in ten serial 1:5 dilutions ranging from 108 to 100 copies per well. Eight replicates were run for each dilution. Results on the TL-988 System show excellent reproducibility and resolution down to very low copy numbers.
Specifications
Thermal Block
Block formats 96-well
Sample volume 5–50 μL
Consumables 0.1ml 96-well unskirted Plates; 0.1ml 8-strip tubes (Optical flat cap)
Max heating/cooling rate 4.2°C/sec
Temperature range 4.0–99.0°C
Temperature accuracy ≤±0.1℃
Temperature uniformity ≤ ±0.3°C
Temperature steps 1-99
Heated lid
Temperature range 100–120°C
Control Automatic temperature and pressure setting
Optics
Excitation 4 LEDs
Excitation range 470–630 nm
Detector Photodiode
Detection range 525–670 nm
Detection channels:
Channel Excitation (nm) Emission (nm) Pre-Calibrated Dyes
1. 470 525 FAM, SYBR Green I
2. 523 564 VIC, HEX, TET, JOE
3. 571 621 ROX, TEXAS-RED
4. 630 670 CY5
Multiplex Up to 4 targets
Detection range 101-1010 copies
Software
Analysis modes: Relative quantification,
Melt curve analysis, High Resolution Melting
Performance
Reproducibility CV≤2.1%
Correlation coefficient of nucleic acid precision R≥0.997
System
Operating systems Windows XP
Communication Ethernet or USB
Power usage AC220 × (1±10%)V, 50 × (1±2%)Hz
Dimensions 540W×300D×480H mm
Weight 35kg
Contact Us
- Shanghai Drawell Scientific Instrument Co., Ltd.
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