Home > Products > Microorganism detecting products > FilmplateTM E.coli&coliform count plate
FilmplateTM E.coli&coliform count plate
- 0 Set / Sets per Month
- T/T L/C D/P D/A
- 7 days
Product Details
Place of Origin: | China | Model Number: | BE203 |
Product Description
1. Principle & application scope
E. coli and coliform which exist in intestinal and feces of animal can cause water pollution and food poisoning. FilmplateTM E. coli & coliform count plate which consist of selective medium, soluble bibulous gel, galactosidase and glucuronidase could test E. coli and coliform at the same time. E. coli shows blue color while other coliforms show red color after growing on the count plate. This product is suitable to detect total coliform number in food materials such as milk drinks and condiments, hygiene detection of instrument surface.
Executive standard: Microbiological examination of food hygiene: Enumeration of Escherichia coli——PetrifilmTM count plate method (GB/T 4789.38)
2. Using method
2.1 Sample preparation: Prepare 1:10 dilution by placing 25mL (g) of sample into 225mL of sterile phosphate buffer solution or sterile saline, and then prepare 1:100 dilution by sucking 1mL of 1:10 dilution into 9mL sterile saline. Prepare tenfold increasing serial dilution of sample if necessary.
2.2 Inoculation: Select 1 or 2 diluted solution to do the test for ordinary sample, while for liquid sample which has little coliform, directly suck the original sample to do the test. Place the FilmplateTM E. coli & coliform count plate onto sterile experiment table; uncover the film on the surface. Suck 1mL of solution with sterile pipette, and then drip it slowly and evenly onto the count plate. Then cover the film gently and stand it for 10s to let the medium solidify. Inoculate two count plates for each different diluted sample solution. At the same time do a blank control.
2.3 Culture: Superimpose the count plates together and place them back to the original ziplock bag, and seal it well. And then put it in the incubator of 36℃±1℃ for 15-24hs with the transparent side up. The quantity of superimposed count plates should not be more than 12.
3. Results
E. coli shows blue color while other coliforms show red color after growing on the count plates.
4. Counting method
4.1 Select the plates of which the quantity of coliform is between 15CFU and 150CFU to do the counting.
4.2 If the colony numbers on count plates from two different dilutions are both between 15CFU and 150CFU, multiply the average number of colonies by dilution multiple as result data.
4.3 If the colony numbers from all the different dilutions are less than 15CFU, count the average number of colonies on count plates of the minimum dilution, then multiply it by the minimum dilution multiple as result data. If no colony was found on all the count plates, then report the result in terms of less than one multiply the minimum dilution multiple.
4.4 If the colony numbers on count plates from all the different dilutions are more than 150CFU, count the average number of colonies on count plates of maximum dilution, then multiply it by the maximum dilution multiple as result data. Or count colonies in one or two squares having representative colony distribution to get the average number of colonies per square, and then multiply this number by 20 (the count plate area is 20cm2) as report data.
Use CFU/mL or CFU/g as report unit.
5. Additional explanatory note
5.1 The PH value of sample solution should be between 6.5 and 7.5, adjust the PH value with 1 mol/L NaOH or 1mol/L HCl if necessary.
5.2 Coliform is usually Chinese food hygienic detection index, while in Europe, E. coli is the food hygienic detection index. Therefore, this product has wide application prospects in area of export food and aviation food.
6. Preservation condition
This kind of product should be placed in the fridge at 4℃-10℃, and the expiration time is one year. Once the package is opened, those unused count plates should be placed back to the package and then seal it well. Put them in the fridge and exhaust them within one month. Condensation may occur in high humid environment, it is better to warm the package to room temperature before unfolding it.
Contact Us
- Guangdong Dayuan Oasis Food Safety Technology Co.,Ltd
- Contact nameMs Sharon Shaw
- Phone86-20-32096888
- Address9/F, NO. 7, Kehui Street 1 ,Kehui Development Center, Science Avenue, Guangzhou Science City, Luogan Baoji,Shaanxi
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