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FilmplateTM water coliform / fecal coliform count plate
- 0 Set / Sets per Month
- T/T L/C D/P D/A
- 7 days
Product Details
Place of Origin: | China | Model Number: | BD105 ( Ⅰ ) |
Product Description
1. Application scope
This product is suitable for rapid detection of coliform and fecal coliform in surface water, domestic sewage, waste water from industries such as medical institutions and poultry breeding industries.
The limit of detection is 20MPN/L.
2. Terms and definition
The below terms and definition are suitable for this kind of count plate.
2.1 Total coliforms
They are defined as rod-shaped, Gram-negative, non-spore forming and motile or non-motile bacteria which can ferment lactose with the production of acid and gas when incubated at 35–37℃ within 24 hours.
2.2 Fecal coliforms
Fecal coliform are facultatively anaerobic, rod-shaped, gram-negative, non-sporulating bacteria which generally originate in the fecal of warm-blooded animals and produce acid and gas from lactose within 24 hours at 44.5℃.
3. Principle
Lactose, indicator (bromcresol purple and 2, 3, 5-triphenyltetrazolium chloride, TTC), nutrient medium are fixed on the count plate. When bacterium breeds, it ferments lactose to produce acid and thus reduces the PH value, and bromcresol purple indicator will turn from purple to yellow. At the same time, dehydrogenase catalyze substrate to reduce TTC (white color) to red insoluble TTF (1, 3, 5-triphenylformazan) during aerogenesis, so red spots are shown on yellow background. According to the color variation, determine whether there is production of acid and gas, thus determine whether there are total coliforms or fecal coliforms. The concentration value of total coliforms or fecal coliforms can be found in MPN table.
4. Materials and reagents
Use national standard analytical reagent unless otherwise stated.
4.1 Sterile water:
Autoclave the fresh deionized water or distilled water at 121℃ for 20 minutes.
4.2 Sodium thiosulfate solution: ρ (Na2S2O3) = 0.10g/mL
Weigh 10g sodium thiosulfate, dissolve it with moderate sterile water or deionized water, and then dilute it to 100mL. Prepare the solution before using it.
4.3 Ethylene diamine tetraacetic acid disodium salt (EDTA-Na2) solution:
ρ (C10H14N2O8Na2·2H2O) = 0.15g/mL
Weigh 15g EDTA-Na2, then dissolve them with moderate sterile water or deionized water, and then dilute it to 100mL. Best use the solution within 30 days.
5. Equipment
5.1 Constant temperature incubator: 37℃±1℃
5.2 Constant temperature incubator: 44℃±0.5℃
5.3 High pressure steam sterilizer: 121℃, 101.3KPA
5.4 Fridge: 0-4℃
5.5 Pipette: 1±0.01mL, 10±0.1mL
5.6 Test tube: Φ15mm × 150mm
5.7 Sampling bottle: 500mL.
Note 1: Sterilize the vitreous utensils such as pipette, test tube and sampling bottle according to the requirement of sterile operation.
Contact Us
- Guangdong Dayuan Oasis Food Safety Technology Co.,Ltd
- Contact nameMs Sharon Shaw
- Phone86-20-32096888
- Address9/F, NO. 7, Kehui Street 1 ,Kehui Development Center, Science Avenue, Guangzhou Science City, Luogan Baoji,Shaanxi
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