Home > Products > Microorganism detecting products > FilmplateTM E.coli STEC count plate
FilmplateTM E.coli STEC count plate
- 0 Set / Sets per Month
- T/T L/C D/P D/A
- 7 days
Product Details
Place of Origin: | China | Model Number: | BS212 |
Product Description
1. Principle & application scope
E. coli STEC can produce Non O157:H7 Escherichia coliform, including E. coli O111 appears in Japan and E. coli O104 appears in Germany and other countries in Europe. This kind of E. coli which parasitizes cows, sheep and other animals can cause diarrhea, colitis and uremia. There are 60 kinds of STEC serotypes are found all over the world, and some non O157 are verified that they are relevant to some food-borne disease. Therefore, these kinds of microorganism should be paid attention to and supervised. FilmplateTM E. coli STEC count plate which adopted imported selective medium and specific enzyme chromogenic reagent is a kind of disposable rapid test product that can greatly simplify detection process and the result can be got after culture for 15-24hs. It is suitable for inspection institutes and food industries.
2. Using method
2.1 Sample preparation: Prepare 1:10 dilution by placing 25mL (g) of sample into 225mL sterile saline. Then prepare 1:100 dilution by sucking 1mL of diluted sample (1:10 dilution) with sterile pipette into 9mL sterile saline.
2.2 Inoculation: Place the FilmplateTM E. coli STEC count plate onto sterile experiment table; uncover the film on the surface. Suck 1mL solution with sterile pipette, and then drop it slowly and evenly onto the plate. Then cover the film gently and stand it for 10s to let the medium solidify. At last press it lightly by hand. Inoculate two count plates for each different diluted sample, and do a blank control test.
2.3 Culture: Pile up the plates together and place them back to the ziplock bag and seal it well. Place it in the incubator with transparent side up. The quantity of superimposed count plates should not be more than 12. The temperature of culture is 36℃±1℃, and the time of culture is 15-24hs.
3. Results
E. coli STEC would show purplish red while other coliforms would show blue after cultivation. The sample which shows positive result should be verified by other detection methods or take the sample to do at least one more test using E. coli STEC count plate.
4. Additional explanatory note
Pay attention that used count plates should be disposed timely according to biology safety waste treatment rule because there are live bacteria on them.
Contact Us
- Guangdong Dayuan Oasis Food Safety Technology Co.,Ltd
- Contact nameMs Sharon Shaw
- Phone86-20-32096888
- Address9/F, NO. 7, Kehui Street 1 ,Kehui Development Center, Science Avenue, Guangzhou Science City, Luogan Baoji,Shaanxi
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