Trichothecenes (T-2) ELISA Test Kit
Trichothecenes(T-2) ELISA Test Kit
Catalog No. LSY-10035
1. General
T-2 toxin is a mycotoxinproduced by various Fusarium. It mainly pollutes wheat, barley, corn and
other food crops andtheir products, which constitute a great hazard to human health and animal
husbandry. T2 mainlyaffects the function of the blood, liver, kidney, pancreatic muscle and
lymphocyte. The clinicalsymptoms of T2 toxin poisoning is anorexia, vomiting, diarrhea, growth
retardation,reproductive and neurological dysfunction etc.The use of Trichothecenes(T2)ELISA
test kit to analyze T2residue of samples is fast and accurate.
This kit is a newgeneration of fungal toxins detection products in application of ELISAtechnology
and the operation timeis shorter than 60min, to minimize operating errors and work intensity.
2. Principle
This test kit is basedon the competitive enzyme immunoassay for the detection of T2 in the sample. Thecoupling antigens are pre-coated on the micro-well stripes. The T2 in thesample and the
coupling antigenspre-coated on the micro-well stripes compete for the anti-T2 antibodies. Afterthe
addition of the enzymeconjugate, the TMB substrate is added for coloration. The optical density
(OD) value of the samplehas a negative correlation with the T2 in it. This value is compared to the
standard curve and theT2 concentration is subsequently obtained.
3. Application
This test kit can beused to detect T2 in corn, rice, wheat, beans, peanuts, oats, feed and other
samples qualitativelyand quantitatively.
4. Technicalspecifications
Sensitivity: 0.2 ppb
Detection limit:
Peanuts, corn, oats,beans, feed etc. …………………………………………………24ppb
Wheat……………………………………………………………………………………..20ppb
Recovery rate:
110±15%
Coefficient of variation: <10%
5. Components
1) Micro-well strips: 12strips with 8 removable wells each
2) 6 x Concentratedstandards………………………………………………………1ml each
0 ppb (zero standard), 2ppb, 6 ppb, 18 ppb, 54 ppb, 162 ppb
Standard workingconcentration: 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb
3) T2 Enzyme-labledconjugate …………………………………………………..… 6ml
4) T2 Antibody…………………………………………………………………….……6 ml
5) SubstrateA.......................................................................................................6ml
6) SubstrateB.......................................................................................................6ml
7) Stopsolution......................................................................................................6ml
8) Concentrated washbuffer(10×)................................................................40ml
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