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LincomycinELISA Test Kit
Catalog No. LSY-10045
1. Principle
Thetest kit is based on the competitive enzyme immunoassay for the detection of Lincomycinin the sample. The coupling antigen is pre-coated on the micro-well stripes. TheLincomycin in the sample and the coupling antigenspre-coated on the micro-well stripes compete for the anti-Lincomycin antibodies. After the addition of the enzyme conjugate, theTMB substrate is added for coloration. The optical density (OD) value of thesample has a negative correlation with the Lincomycin in it. Thisvalue is compared to the standard curve and the Lincomycinresidues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Tissue.......................................................................................... 0.4ppb
Feed............................................................................................... 1ppb
Honey............................................................................................. 2ppb
Cross-reactionrate
Lincomycin...................................................................................... 100%
Recoveryrate
Tissue………………………………………………………………….100%±25%
Feed............................................................................................ 95±25%
Honey..................................................................................... 100%±25%
3. Components
1） Micro-well strips: 12 strips with 8 removable wellseach
2） 6× standard solution (1 mL each): 0 ppb, 0.1ppb, 0.3ppb, 0.9 ppb, 2.7 ppb,8.1 ppb
3） Enzyme conjugate (7 mL)............................................................... redcap
4） Antibody working solution (7 mL).................................................. bluecap
5） Substrate A solution (7 mL)......................................................... whitecap
6） Substrate B solution (7 mL)......................................................... blackcap
7） Stop solution (7 mL).................................................................. yellowcap
8） 20x concentrated washing buffer (40 mL) ................................... white cap
9） 20× concentrated sample extraction solution (50 mL) ………………transparentcap
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