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Help Center3-(3-Cholamidopropyl)-dimethylamino-1-propanesulfonic Acid (CHAPS)
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Purity: 98%
Alias: CHAPS;3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonatehydrate;
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, zwitterionic;
Cholamidopropyl-dimethylammonio]-1-propanesulfonate
Biochemical Mechanism:
CHAPS is a zwitterionic, non-denaturing detergent used for the solubilization of membrane proteins.
Storage Temperature: Store at 2–8°C under a nitrogen atmosphere.
Shipping Conditions: Shipped with ice packs.
Features
CHAPS is a non-denaturing, zwitterionic detergent derived from cholic acid. CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM.
A non-denaturing, zwitterionic detergent for membrane proteins
Applications
Buffer; zwitterionic nondenaturing detergent for solubilizing membrane proteins CHAPS is used in the laboratory to dissolve biological macromolecules such as proteins. It acts as a non-denaturing solvent used in purification of membrane proteins. It is also useful in conjunction with nonionic detergents like Triton X-100. Further, it is used as a non-denaturing zwitterionic detergent for membrane biochemistry.
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