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Help Center3-Morpholinepropanesulfonic Acid (MOPS）
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3-Morpholinepropanesulfonic acid (MOPS)
Melting Point: 277–282°C
Density: 1.3168 (rough estimate)
Vapor Pressure: 0 Pa at 25°C
Refractive Index: 1.6370 (estimate)
Flash Point: 116°C
Storage Conditions: Store at 25°C, protected from light.
Solubility: H₂O: 1 M at 20°C, clear
Form: Powder/Solid
Color: White
Odor: Odorless
pH Value: 2.5–4.0 (25°C, 1 M in H₂O)
pH Indicator Color Change Range: 6.5–7.9
Acidity Constant (pKa): 7.2 (at 25°C)
Water Solubility: 1000 g/L (20°C)
Maximum Wavelength (λmax): λ: 260 nm, Amax: 0.020; λ: 280 nm, Amax: 0.015; Merck: 14, 6265; BRN: 1106776
Stability: Stable. Incompatible with strong bases and strong oxidizing agents. InChIKey: DVLFYONBTKHTER-UHFFFAOYSA-N; Log P: -2.94 at 20°C
Key Properties:
1. Efficient Buffer: Excellent buffering range of pH 6.5 – 7.9.
2. Low Interference: Exhibits minimal reactivity with metal ions, making it ideal for studies requiring non-coordinating conditions.
3. High Purity: Available in various grades, including cell culture and bio-performance certified.
4. Limitations: It can interfere with the Folin protein assay and partially decompose if autoclaved with glucose.
Applications
1. Cell Culture: Maintains pH stability in culture media for mammalian cells, bacteria, yeast, and other cell types; typically used at concentrations not exceeding 20 mM.
2. Nucleic Acid and Protein Research: Applications include RNA denaturing agarose gel electrophoresis, protein purification, and electrophoretic separation; serves as a commonly used buffering system for RNA electrophoresis.
3. Biochemistry and Molecular Biology: Functions as a buffering component in various reagent kits (e.g., PCR, DNA extraction); widely utilized in diagnostic reagents and biosynthesis.
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