Drawell 4 Channel Plates Strip Tube 0.1ml Test Kit Machine Gradient Real Time PCR
RT-PCR Gentier 96R
PECIFICATION
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Gentier 48E |
Gentier 48S |
Gentier 48R |
Gentier 96E |
Gentier 96S |
Gentier 96R |
Gentier 96C |
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Thermal Block |
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Sample capacity(wells) |
48
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96
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Reaction Volume |
5-100 pL |
0-100 pL
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Consumables |
0.2ml 8-strip tubes ,0.2ml PCR single tube(Optical flat cap,clear tube) |
0.2ml 96-well Plates |
(unskirt) ; 0.2ml 8-strip tubes,0.2ml PCR single tube(Optical flat cap,clear,frosted,white tube) |
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Temperature range |
0°C-100°C
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0°C-100°C
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Heating/cooling method |
Peltier |
Peltier
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Max heating rate |
8.0°C/sec
|
6.1°C/sec
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Average heating rate |
6.0°C/sec
|
4.5°C/sec
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Max cooling rate |
5.5°C/sec
|
5.0°C/sec
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Average cooling rate |
4.0°C/sec
|
2.8°C/sec
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Temperature accuracy |
±0.1 °C
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±0.1 °C
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Temperature uniformity |
±0.1 °C
|
±0.1 °C
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Gradient Range |
1°C-40°C |
N/A |
1°C-40°C |
1°C-40°C |
N/A |
1°C-40°C |
N/A |
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Gradient block |
8 row |
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8 row |
12 row |
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12 row |
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Special temperature protocol |
Gradient PCRXong PCR,Touch Down PCR |
Long PCR.Touch Down PCR |
Gradient PCRzLong PCR,Touch Down PCR |
Gradient PCR,Long PCRlbuch Down PCR |
Long PCR,Touch Down PCR |
Gradient PCR,Long PCRlbuch Down PCR |
Long PCR,Touch Down PCR |
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Heat Lid |
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Temperature range |
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Room Temperature-110°C |
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Room Temperature-110°C |
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Optical System |
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Excitation Source |
4LEDs |
2LEDs |
6LEDs |
4LEDs |
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Detector |
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Photodiode |
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Photodiode |
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Detection Position |
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Excitation and scan from lateral |
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Excitation and scan at top |
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Detection method |
4 channels scanning at the same time, no edge effect. |
|
6 channels scanning at the |
same time, no edge effect. |
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Detection time |
|
2 seconds for 48 wells for all channels |
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7 seconds for 96 wells for all channels |
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Range of excitation/ emission wavelengths(nm) |
1 465 /510 (FAM,SYBR Green l,SYTO9,EvaGreen,LC Green) 2 527/ 563 (HEX,VIC,TET,JOE) 3 580 /616(ROX,Texas Red) 4 632/ 664( Cy5) |
1 465 /510(FAM,SYBR Green I.SYTO9, EvaGreen,LC Green) 2 527/ 563 (HEX,VIC,TET,JOE) |
1.465 /510(FAM,SYBR Green l,SYTO9,EvaGreen,LC Green) 2.527/ 563 (HEX,VIC,TET,JOE) 3.580 /616 (ROX,Texas Red) 4.632/ 664( Cy5) 5.680 /730 (Alexa Fluor680) 6.465 /616 (FRET) |
1.465 /510(FAM,SYBR Green l,SYTO9,EvaGreen,LC Green) 2.527/ 563 (HEX,VIC JET,JOE) 3.580 /616 (ROX,Texas Red) 4.632/ 664( Cy5) |
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Probe |
Taqman ProbezMolecular beacons probe, Scorpion probe |
Taqman Probe,Molecular beacons probe,Scorpion probe ,FRET |
Taqman Probe,Molecular beacons probe,Scorpion probe |
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Multiplexing |
Up to 4 targets |
Up to 2 targets |
Up to 6 targets |
Up to 4 targets |
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Fluorescence Linearity |
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r>0.990 |
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rs0.990 |
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Fluorescence Dynamic Range |
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Adjustable |
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Adjustable |
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