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Porcine Japanese B Encephalitis Virus Ab ELISA Kit
Catalog No. LSY-30008
1. Usage
The porcine encephalitis virus ELISA test kit is used for the detection of the porcine encephalitis virus antibodies in swine serum; assessment the immunity conditions against porcine encephalitis virus in the pig farms and investigation of the epidemiology of the porcine encephalitis virus.
2. Principle
The kit is based on an indirect enzymatic immunoassay(Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific peroxidase conjugate(IgG-HRP). After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer(630 nm).
3. The kit components
1 Antigen coated microtiter plate2 pieces (96 wells)
2 Negative (green lid)/positive (red lid)serum1 tube each (1.5 mL/tube)
3 Enzyme conjugate (yellow lid)1 bottle (22 mL/bottle)
4 20×concentrated washing buffer (white lid)1 bottle (50 mL/bottle)
5 Substrate A (purple lid)/B (orange lid)1 bottle each (12 mL/bottle)
6 Stop solution(blue lid)1 bottle (12 mL/bottle)
7 Sample diluent solution(transparent lid)1 bottle (50 mL/bottle)
8 Adhesive Foil6 pieces
9 Instruction1 piece
(Note: Never mix up reagents from test kits in different batch number)
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