Foot-and-mouth Disease Virus (FMDV) Type O Antibody ELISA Test Kit
Foot-and-mouth disease virus (FMDV) Type O Antibody ELISA Test Kit
Catalog No. LSY-30031
1. Principle
This kit is used to detect specific antibody against foot and mouth disease virus (FMDV) Type O Antibody in serum of bovine、goat and porcine qualitatively, for monitoring antibody after FMDV Type O immune.
This kit use block ELISA method, FMDV antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is FMD virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-FMD virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.
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