Hepatitis B test kit manufacture

Hepatitis B test kit  manufacture

Enzyme-linked immunosorbent assay for the detection of HBsAg / hbcab / hbeab / hbeag / hbsab in serum or plasma   

The hepatitis b elisa test kits  is used for the qualitative determination of 2-8°C  in human serum or plasma. This test is indicated for the screening of blood and blood products to be used for transfusion and an aid for the diagnosis of existing or previous hepatitis B infection.

The hepatitis b elisa test kits is a solid-phase simultaneous sandwich immunoassay, which employs monoclonal antibodies and polyclonal antibodies specific for HBsAg / hbcab / hbeab / hbeag / hbsab  . Microtiter well is coated with monoclonal antibodies specific for HBsAg.  A serum specimen is added to the antibody coated Microtiter wells together with enzyme conjugated polyclonal antibodies. HBsAg, if present, will form an antibody-HBsAg-antibody-enzyme complex.  The plate is then washed to remove unbound material. Finally, a solution of substrate is added to the wells and incubated. A blue color will develop in proportion to the amount of HBsAg present in the specimen. The enzyme-substrate reaction can be stopped and the result is visualized by naked eye or read by EIA plate reader for absorbance at the wavelength of 450 nm(450 nm/630 nm) and is proportional to the amount of antibodies present in the specimen

Serum should be prepared from a whole blood specimen obtained by acceptable medical techniques. Either serum or plasma can be used in this test.  Remove serum or plasma from the clot or blood cells as soon as possible to avoid hemolysis.  Specimen with extensive particulate should be clarified by centrifugation prior to use.  Specimen frozen at -20°C or colder may be used.  Avoid repeated freeze thaw. 

Unopened test kits should be stored at 2-8°C. DO NOT FREEZE KIT COMPONNETS.  The microtiter plate should be kept in a sealed bag to minimize exposure to damp air. Use up the reagents as soon as possible after the kit is unpacked

Our Services

1.Factory direct price .price is competitive with high quality. 

2. Original manufacturer provide OEM service for you

3. Smooth delivery near beijing and tianjin Port. 

4. Free samples available sent for your evalutaiton.

Company Information

Our company is in Beijing, China beginning built in 1988.Specialized in the field of ELISA TESTS&RAPID TESTS as both supplier and manufacturer with GMP,ISO9001:2008, SFDA and ISO13485 certificates. Our company was the earliest domestic high-tech enterprise which specializes in the research, test, manufacture and sale of genetic engineering drugs and diagnostic reagent.