100L GMP Cell Bioreactor

100L Tank

Nominal volume: 100L

Diameter ratio: 1:1.5-1.8 (according to customer demand design and production); the best loading coefficient: 70%

Tank body: the main body material for the whole stainless steel 316L tank (jacket 304L), no dead corner in the tank; with a special sampling of fermentation tank, discharge valve. Large viewing angle tank liquid level observation mirror, 12V safety light lamp, with temperature, PH, DO, anti foam, liquid level interface, vaccination, feeding port, and a number of spare ports, all of the weld solid and tidy appearance. The pressure gauge which is in contact with the material is the diaphragm type pressure gauge.

Tank: design pressure 0.3MPa jacket: design pressure of 0.3MPa, the optimization design of diversion, improves the switching efficiency and tank temperature uniformity, uniform and stable temperature control is fermentation can be the basic condition for success, tank top with safety of blasting, the maximum reduce the risk probability.

Tank surface treatment: internal and external surface polishing, polishing accuracy of less than Ra0.4; all the welding line is not polished (in accordance with international standards)

SIP

SIP

1, Can achieve the empty can, real tank and air filter manual sterilization (can also be automatic);

2,  The program with Germany Gaimi pneumatic valve a type of automatic control, the maximum improve the sterilization efficiency, save labor cost.

3, Each need to operate the valve when the critical point, there are warning tips and voice prompts

4, Disinfection map can be printed (temperature changes);

5, The end of sterilization, can control the electromagnetic valve of the switch to the sterile air to cool the pipeline, switch time can be set.

Sterilization temperature /

121 degrees Celsius, according to the process need to be able to adjust the settings

Sterilization time

Temperature to reach 121 degrees Celsius after the pressure, the whole process of 60 minutes, time can be set

Culture temperature

PID automatic control, electric heating, water cooling, with circulatory system; control range: cooling water temperature +5~65 DEG C control accuracy + / - 0.2 DEG C, resolution of 0.1 DEG C; low temperature control: can automatically switch to low-temperature hold 4 to 6 DEG C (need to configure refrigerant); with real-time curve and history curve, sectional set of tracking control.

Over limit alarm function

PH

PID automatic control, intelligent automatic control, high control precision, can be used to control the flow of 1-2 units with the control flow of the pump acid and alkali, automatic control, automatic measurement. The electrode can be repeatedly sterilized, and the correction function is completely corrected by the system software.

The wire electrode and glass gel imported from Switzerland are resistant to high temperature disinfection;

Automatic control range: 2.00-12.00 + 0.02, control accuracy of 0.1;

Online detection, detection accuracy: 2.00-12.00 + 0.01;

Automatic alarm, automatic adding and adding acid and alkali liquor;

Intelligent fuzzy control.

Controller: pH curve analysis, acid, and alkali curve analysis, batch statement analysis, acid addition amount of cumulative display records, alkali amount accumulative total display and recording, order of fermentation process control (according to the time of fermentation, dissolved oxygen (do) automatic control variables, at least 10 section), data security deposit.

Over limit alarm function

DO

PID automatic control, adopts the intelligent automatic control, high control precision, air to flow through the rotor flowmeter manual control or mass flowmeter automatic control and control of stirring velocity correlation, both by changing the stirring speed adjusting the dissolved oxygen, control range: 0~250%, the control accuracy: + - 1 DEG C; resolution: 1%, with real-time curve and history curve, and speed, feeding and other parameters were associated with the control.

Measurement range 0.1-200%, control accuracy: +/- 1%, resolution: 0.1%;

Fermentation process sequence control (according to the fermentation time, DO automatic variable control, at least 10 sections).

Over limit alarm function

Reactor pressure control

Manual control, diaphragm type pressure gauge auxiliary display, control range: 0-0.4MPa, automatic pressure control, with the electronic pressure sensor, pneumatic valve and mass flow meter automatic control, the screen can display and calibration.

Stirring system

PID automatic control, the control speed governor can and do correlation;

Can carry out at least 10 speed setting and automatic setting control;

Using strong magnetic mechanical suspension magnetic stirring system (full seal, zero leakage, no pollution, will be changed to static seal, completely solve the problem of biological products tank culture and bacteria);

The low shear force and the height of the 2 grade three leaf blade are low shear force and high degree. The contact part of the material is 316L

Control range: 100L, 0-300rpm, control accuracy: +/- 1%, resolution: 1rpm

Can be related to the control of dissolved oxygen, with real-time curve and history curve, can be set up tracking control;

Speed value report, curve record and analysis, data storage, and transfer to the host computer.

Over limit alarm function

Ventilation system

Our software can be a good fusion of mass flow meter, control the volume of air to achieve the accuracy of customer requirements.

Air 1:0 ~ 30L/ minutes

Air 2:0 ~ 30L/ minutes;

O 2:0 ~ 6L/ minutes;

Carbon dioxide: 0 ~ 6L/ minutes;

Accuracy: +/- 1%, resolution: 0.1%

Can be associated with the DO and tank pressure control;

Over limit alarm function

Material feeding system

Perfect compensation system and can be timing quantitative feeding; each tank four peristaltic pump can respectively in the control interface of block set, in actual use is convenient and flexible, configuration, high temperature resistant repairing material bottle and filter, 316L feeding needle.

With digital timing quantitative piecewise control settings, can control the peristaltic pump feeding automatic control, according to the requirement of the level, we set the control of feeding, feeding can and dissolved oxygen associated control.

The controller can be realized: the feeding volume curve analysis, the amount of adding amount accumulated, display records, batch report analysis data storage.

Can be customized according to user needs different flow rate of the pump, the function of the pump can be set on the control interface.

Defoaming

Using foam detection sensor, control accuracy: 10-100000Ω; automatic detection of foam or liquid level, can be used to automatically add the foam agent or the filling material, and metering, automatic control.

Over limit alarm function

On-line sampling

Special online sampling valve: professional design and manufacture, simple structure, no dead angle, steam sterilization, easy to use

Tank lid lifting

BLBIO-VIP

Imported lifting assembly with safety lock dead function, safe and reliable, convenient cleaning, Shanghai dimensional tank parts.

Tank weighing

Teller weighing module for automatic weighing and the volume of fermentation liquid, precision: one thousandth ,range: 0-150Kg, online detection of the fermentation liquid volume (weight), output signal (load cell, Switzerland Mettler brand)

Program rights management

Program rights management (four levels)

1, The operator: only basic operation (browsing, the operation of the valve)

2, Super user: basic operation and basic parameters of the modification

3, System administrator: the modification of the background parameters

4, The administrator: all the parameters of the equipment modification, the authorization of all management rights

The highest authority to display and modify all parameters, each level can only display specific permissions, the other can not be displayed

Data collection and alarm

1 Capable of generating temperature, pH, stirring speed, dissolved oxygen parameters such as curve and report.

2 Can save at least 100 batches or more than one year of fermentation data, and can refer to the history.

3 When temperature, pH, speed and oxygen detection value exceeds the set range, can send alarm.

4 Record function:

--Per level personnel's landing and everyone's operation record

--System log.

--Alarm information.

--Each project of the record information can not be modified, automatically saved, can be arbitrary query.

Safety protection of tank

1, When the control system power failure occurs, the device immediately stop running, protect the operator, equipment and products, to restore the power supply, by the operator to restart the operation, the device does not automatically run;

2, The safety performance of the power system is in line with the corresponding national standards;

3, The equipment has the function of safety protection, when the safety protection device is not in the normal state, the device stops running, and displays the corresponding alarm information on the operation interface;

4, Equipment noise is lower than the national standard;

Control software,Intelligent man-machine interface in English

Control system: four sets of fermentation tank independent controller, can also use a controlled multi integrated controller.

Field controller, the lower position machine for Siemens s7-1200 PLC control system, it is a mature, stable, a generic type of PLC, can be suitable for various application of automation, especially manufacturing process control in the application. The modular and easy to easy to grasp the characteristics and the realization of distributed configuration, the Siemens PLC control core has become the implementation of bio reaction process control in both economic and advanced control system, system adopts touch screen display, on-site direct manipulation (menu), full Chinese menu single and interface; can also with computer for data transfer, sampling and data display tank, analysis of system data browsing, set of readings.

Using 15 inch integrated LCD touch screen as a display interface, display the contents of a rich and complete, user-friendly picture is simple and clear, easy to operate. And do not interfere with each other. The control system can adapt to different sensor and actuator range of the output input signal, with the signal anti interference system.

Control and display parameters: temperature, stirring speed, pH, dissolved oxygen, fire bubble, air flow and the pressure in the pot, fermentation liquid volume and exhaust O2, CO2 exhaust, 14 direct parameter and indirect calculation parameters.