Ebola kits (fluorescence PCR method)

Specifications

can dedect Ebola virtues within 3 to 4 hours based fluorescence PCR method

Ebora virus nucleic acid determination kit (fluorescence PCR method)

 

Ebora virus nucleic acid determination reagent kit used in fluorescent PCR platform, on the Ebora virus were determined.

 

Ebora (Ebola) is a general term used to address a group belongs to the fiber virus, Ebora virus under several kinds of virus, composed of negative strand RNA virus particle chain, can lead to Ebora viral hemorrhagic fevers, mortality is 50% to 90%. Mode of transmission of airborne infectious body fluids of an infected person, (can only survive for a period of time).

 

Nucleic acid type: RNA

QR-0220-01

QR-0220-01

I, II

I, II

(I)LightCycler,

(I) LightCycler,

(II)LightCycler2.0

(II) LightCycler2.0

QR-0220-02

QR-0220-02

III,IV

III, IV

(III) P5700,MJ-Option

(III) P5700, MJ-Option and other monochrome fluorescent PCR instrument

 

(IV) ABI7000, AB7300, ABI7500, ABI7900, LightCycler480, MJ-, Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor- Gene3000, iCycler IQ4, iCycler IQ5, CF96 multicolor fluorescence PCR instrument

 

Ebora virus is a highly dangerous pathogens, isolation and identification of virus in laboratory facilities must be carried out within specialized. In Africa epidemic mainly through the detection of the Ebola virus specific IgM and IgG antibodies and detection of virus antigen or nucleic acid diagnosis.

 

The virus specific antibody check

 

Virus specific IgM antibody in the blood of patients in 2 ~ 9 days after onset, 1 ~ 6 months after the onset of persistent to; IgG antibodies in the pathogenesis of after 6 ~ 18 days appear, continue to exist up to more than 2 years after the onset of. Gene engineering preparation method of the viral core protein carboxyl terminal peptide as antigen, ELISA method for detection of Ebola virus IgG antibody of the establishment, with high sensitivity and specificity. But for some patients with acute phase serum specific antibody titer is low, should be detected simultaneously virus antigen or nucleic acid.

 

The virus specific antigen and nucleic acid examination

 

Has confirmed the consistency detection of Ebola virus antigen and detection of viral nucleic acid reached almost 100%, the sensitivity is high. And, with the? Ray irradiation specimen and inactivated virus, and then detecting virus antigen or RNA, experimental safety increased, and the experimental results are not significantly affected by.