Cell Culture Fermenter/jacketed bioreactor/Fermentation tank/Pharmaceutical fermentation/Situ fermenter/Industry pilot fermentor

Equipment

Item

70L fermenter

Working volume:10L to 50L

Fermenter body

The size of fermenter takes in general use type as basis and The main material of the fermenter is made by 316Lstainless steel. The design pressure: P(inside)= 0.3Mpa, P(interlayer)= 0.3Mpa, The polishing precision: Ra0.4~0.6, the ratio between diameter and height: 1:2.2 

70L fermenter   Working volume:20L to 50L ,

300L fermenter  Working volume:50L to 210L

Sterilization method

Auto sterilizing in situ

Sterilization method

Magnetic Drive from bottom, without mechanical seal
The agitation system hanged up is driven by magnetic force. There is no thimble at the bottom. The rotate speed is steady. High-powered stirrer, antifoam stirrer, AC buncher, variable speed control. All parts in the cylinder are set on the cover, so it is convenient to maintain and clean.

Temperature detection and control

Heat by thermostatic water tank and circulate by circulating pump(Grundfos, Denmark), autocontrol (cooling water+5℃)~70℃±0.1℃,temperature probe(imported)

pH detection and control

2.00-12.00±0.05pH, autocontrol by adding acid and base, pH sensor (Hamilton /Mettler,Switzerland) and shielding lead (Hamilton /Mettler, Switzerland). 30L fermenter is autocontrolled with   peristaltic pump

DO detection and control

0-150±3% displaying precision 0.1%, sensor (Hamilton /Mettler, Switzerland) and shielding lead(Hamilton /Mettler,Switzerland)

Feed control

fermenter uses peristaltic pump (Longer) to add meterials

Antifoam control

Tested by sencor and added antifoamer by peristaltic pump

Aeration detection and control

 the precision of inhaler : 0.1μ, displayed by rotameter.  Gas Supplement : Air, O2, N2, CO2

pressure detection and control

Auto  control exhaust by diaphragm cell valve,and displayed by pressure gauge.

Pipeline and valve

The pipelines and valves which are in contact with the zymotic fluid are made by 316L stainless steel, the valves are diaphragm cell valves

Control system

BLBIO S Control System: on-site controller, the next crew for the Siemens S7-300 series PLC control system, it is a mature, stable, universal type of PLC, to suit a variety of automation applications, especially in manufacturing process control Application. Its modular, easy to implement distributed configuration and easy to master and so on, Siemens PLC control center has become a biological reaction process control implementation is economical and advanced control system, the system uses the touch screen display, on-site direct operations (menu type), all English menu and interface; also with the host computer for data transmission, data sampling and display tank, the system view of data analysis, reading settings.

10-inch LCD touch-sensitive screen as the display screen to display complete content-rich, user-friendly screen is simple, easy to operate. And disturbing each other. The control system can adapt to different range of sensors and actuators the output of the input signal with the signal anti-jamming system.

Software: Siemens S7+300  FORCE  CONTORL

Data collection and control module: Siemens S200 PLC controller and data collection and control module

1. Control
1) Manual control: You can set the percentage of open valve or a valve opening 2) automatic control mode: You can select continuous or PID PID control the switch 3)sequence control: the control of all parameters can be pre-set at least 10 control section, 4) for automatic segmentation remotely control: PC control  5) acceptable correlation control: the dissolved oxygen can select speed, air flow rate, tank pressure, control of feeding, etc.; pH can choose to control with acid and alkali

2. Measurement function: can feeding, bubble enemy, such as measurement of pH
3. XY-axis to chart real progress can be any expansion and contraction of graphics display, to help the operator determine the parameters of the interaction between the effects of the fermentation process to quickly modify and adjust

4. Data Processing
1) to show that all the parameters of the trend curve analysis
2) to store, display, analyze all the historical curve parameters
3) can display all the parameters of the batch report
4) copy the data directly with the USB

5) data storage format is compatible with EXCEL, EXCEL platform can be to deal with
5. Controller self-protection function: to set the password, others can not modify the fermentation parameters; and the controller is not lost due to power settings for each parameter
6. Can the process control parameters, each parameter can have 10 block can be set
7. Time display of each parameter recording interval can be adjusted
8. Two parameters can be carried out with the screen settings and calibration

9. show the controller is really running against, the panel instructed the state process system, process hardware, and motion status indicator for understanding the various devices (such as peristaltic pumps, circulating pumps, heating, mixing, etc.) work status, Fault identification can be used as display
10. Each parameter of the transmission are independent modules, without disturbing each other, for easy maintenance
11.PH, DO and other parameters of control and associated controls can be adjusted

No

Parameter

Guideline

Controller data processing function

1

pH

Displaying range: 0.00~14.00±0.01

Auto-control range:2.00~12.00±0.05

Fermentation process control (According to the fermentation time, auto- control pH fluent, at least10 parts)

pH value historical and trend curve analysis

Acid, alkali dosage curve analysis

Batch report form analysis

Acid, alkali total dosage record

Keep and deliver data

2

DO

0-150±3%,Displaying precision: 0.1%

It can be controlled by rev and feeding.

Fermentation process control (According to the fermentation time, auto- control DO fluent, at least10 parts)

DO value historical and trend curve analysis

Batch report form analysis

Keep and deliver data

3

Antifoam

Automatic PID control and alarm

Fermentation process control (According to the fermentation time, auto- control antifoam fluent, at least10 parts)

Antifoam value historical and trend curve analysis

Foam error state record

antifoam total dosage record

Batch report form analysis

Keep and deliver data

4

Rev

Automatic enactment and control

Fermentation process control (According to the fermentation time, auto- control rev fluent, at least10 parts)

Rev value historical and trend curve analysis

Batch report form analysis

Keep and deliver data

5

Temperature

Temperature of cooling water: +5℃-70℃±0.1℃

Fermentation process control (According to the fermentation time, auto- control temperature fluent, at least10 parts)

Temperature value historical and trend curve analysis

Batch report form analysis

Keep and deliver data

6

Feeding

PID automatic enactment and control

Fermentation process control (According to the fermentation time, auto- control feeding fluent, at least10 parts)

Feeding value historical and trend curve analysis

Feeding total dosage record

Batch report form analysis

Keep and deliver data

7

Pressure

Auto  control, instrument display

8

Air flux

Auto   control, instrument display

No.

Name

Descrition

Brand

Country or Area

1

Tank

Main body material use 316L+304 clamp

Bailun

China

2

Stirring motor

SEW

SEW

Germany

3

Speed regulator

Fuji

Fuji

Japan

4

Site control system

Germany's Siemens S7-300 series PLC control system + 10 inch touch screen +control circuit board+ electrical box and various appliances, including cabinets, control core, signal acquisition, transmitter, power drive and various type of actuator

Bailun

China

5

Flow meter

 200L/min

Chengfeng

China

6

Air intake precise filter

5 inch,0.2μm

Sartorius

Germany

7

Exhaust gas precise filter

5 inch,0.2μm

Sartorius

Germany

8

Halitus filter

Sartorius φ25

Sartorius

Germany

9

Feeding peristaltic pump

BL-0~150mlmin

Spirax

UK

10

Acid feeding

peristaltic pump

BL-0~60mlmin

Spirax

UK

11

Alkali feeding peristaltic pump

BL-0~60mlmin

Spirax

UK

12

Anti-foaming pump

BL-0~30mlmin

Spirax

UK

13

 PH Electrode

Originally imported from Switzerland 120

Switzerland

Switzerland

14

PH Electrode

Originally imported from Switzerland 3M

Switzerland

Switzerland

15

DO Electrode

Originally imported from Switzerland 120

Switzerland

Switzerland

16

DO Cable

Originally imported from Switzerland 3M

Switzerland

Switzerland

17

Temperature Electrode

PT100

burkert

Germany

18

Bearing

SKF

FKF

Sweden

19

Piping

316L Stainless steel

China

20

Other parts

Feeding bottles, silicone tube, piping, pipe racks, various types of valves, Pressure parameter, bearing, sealing, tools, etc.

Imported from Europe

Europe

21

 Pressure ssensor

-0.1—0.3Mpa,sanitation

burkert

Germany

22

Control valve

Pneumatic angle seat valve for drainage control

burkert

Germany

23

Control valve

Pneumatic angle seat valve for isolation control

burkert

Germany

24

Control valve

Pneumatic angle seat valve for cooling control

burkert

Germany

25

Control valve

Pneumatic angle seat valve for sterilization control

burkert

Germany

26

Pneumatic diaphragm valve

For gas control, etc.

burkert

Germany

27

Exhaust control valve

ON/OFF

burkert

Germany

28

Pilot valve

Control pneumatic valves

burkert

Germany

29

Diaphragm valve

Feeding system sterilization

burkert

Germany

30

Manual diaphragm valve

For sampling systems

burkert

Germany

31

Pneumatic diaphragm valve

For air intake and exhaust system

burkert

Germany

32

Hydrophobic valve

Release condensate water for sterilization, share with system

Spirax

UK

33

Circulation pump

Grundfos

Grundfos

Denmark